This article released onMiniTool official sitegives official and latest answers for the standard and animated Twitch emoji sizes.
Besides, it also provides music broadcasts, creative content, and the recent in real life streams.
Yet, no matter who creates the emote, they all should obey some rules like below.

What Size Is a Twitch Emote?
(Standard)
A Twitch emote size depends on how you upload it to the platform.
All in all, the emote file size mustnt exceed 1MB.

What Is the Size of a Twitch Emote?
1MB file size maximum for auto-resize mode and 512KB max for manual upload mode.
#1.
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They are including but are not limited to the following ones.
What Discord Emoji Size is?
How to access Discord emotes?
How to add custom Discord emojis?
Want to learn more pro tips about Discord emoji?
#2.
You may be asked to take down the involved emotes or even face a lawsuit.
The following are some examples of content that you shouldnt make use of without authorization or permission.
Another person or organizations name, logo, as well as other identifying marks.
The likeness of another person, such as the face of a celebrity.